The best Side of how HPLC works
The best Side of how HPLC works
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To forestall the lack of stationary period, which shortens the column’s life time, it is certain covalently to your silica particles. Bonded stationary phases
각각 다른 산업 분야에 대한 자세한 정보 및 다양한 카테고리는 다음 써모 피셔 사이언티픽 학습 센터에서 산업 및 응용 과학 페이지를 확인하세요.
物質の濃度により光の通過する角度が変わることを利用した検出器。原理上グラジェント分析はできない（グラジェントによって移動相自体の屈折率が変化するため）。また、感度が低いのが欠点だが、大部分の物質に対して使用できる。
). Since the tubing and fittings that carry the mobile period have force restrictions, a higher back again strain needs a decrease circulation level and a longer Assessment time. Monolithic columns, by which the sound assistance is a single, porous rod, supply column efficiencies comparable to a packed capillary column although allowing for more quickly move rates. A monolithic column—which commonly is similar in dimensions to a traditional packed column, While smaller, capillary columns also can be obtained—is prepared by forming the mono- lithic rod in a mold and masking it with PTFE tubing or perhaps a polymer resin.
are made by reacting the silica particles using an organochlorosilane of the overall form Si(CH3)2RCl, the place R is check here an alkyl or substituted alkyl team.
テキストはクリエイティブ・コモンズ 表示-継承ライセンスのもとで利用できます。追加の条件が適用される場合があります。詳細については利用規約を参照してください。
-hydroxybenzoic acid (PH) on the nonpolar C18 column subject matter to a utmost analysis time of six min. The shaded parts signify areas wherever a separation is impossible, While using the unresolved solutes discovered.
The pump is the heart of the HPLC system. It provides the mobile section at a relentless and high force (around four hundred atm) with the column. Reliable circulation price is significant for obtaining ideal separation and preserving reproducibility. Factors to look at when deciding on a circulation rate include things like:
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Retention instances: The time it will require for each analyte to get to the detector, delivering a attribute fingerprint for identification.
Measurement-exclusion chromatography, also referred to as gel filtration or gel permeation chromatography, separates substances based on their dimensions and molecular fat. Lesser molecules can penetrate the porous framework in the stationary section and elute speedier, although much larger molecules are held for a longer time.
In the fuel chromatograph the pressure from a compressed fuel cylinder is sufficient to press the cellular section throughout the column. Pushing a liquid cellular section through a column, nonetheless, takes an incredible deal far more work, building pressures in excessive of a number of hundred atmospheres.
검토 중에서 컬럼이나 이동상 등 여러 조건의 조합은 분석 가능성의 큰 영향을 미칩니다.)
Along with the analysis procedure comprehended, let's deal with popular troubles which could crop up and how to troubleshoot them.